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brightline filters af488  (IDEX)

 
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    Structured Review

    IDEX brightline filters af488
    a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing <t>DAPI</t> (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d-f Similar diagrams and images as (A-C), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g-h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Real data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and 2D KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’. i-n Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). Solid line is the mean and shaded area is the standard deviation. Dashed lines is the background fit. Dotted line is the colocalization by chance. i, k, m Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (i), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (k), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (m). A total of n = 651 cells are quantified in this analysis. j, l, n Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (j), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (l), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (n). A total of n = 625 cells are quantified in this analysis.
    Brightline Filters Af488, supplied by IDEX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/brightline+filters+af488/bio_rxiv__2025__01__05__631290-270-6-5?v=IDEX
    Average 90 stars, based on 1 article reviews
    brightline filters af488 - by Bioz Stars, 2026-07
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    Images

    1) Product Images from "Transcriptional stochasticity reveals multiple mechanisms of long non-coding RNA regulation at the Xist – Tsix locus"

    Article Title: Transcriptional stochasticity reveals multiple mechanisms of long non-coding RNA regulation at the Xist – Tsix locus

    Journal: bioRxiv

    doi: 10.1101/2025.01.05.631290

    a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing DAPI (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d-f Similar diagrams and images as (A-C), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g-h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Real data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and 2D KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’. i-n Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). Solid line is the mean and shaded area is the standard deviation. Dashed lines is the background fit. Dotted line is the colocalization by chance. i, k, m Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (i), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (k), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (m). A total of n = 651 cells are quantified in this analysis. j, l, n Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (j), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (l), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (n). A total of n = 625 cells are quantified in this analysis.
    Figure Legend Snippet: a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing DAPI (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d-f Similar diagrams and images as (A-C), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g-h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Real data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and 2D KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’. i-n Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). Solid line is the mean and shaded area is the standard deviation. Dashed lines is the background fit. Dotted line is the colocalization by chance. i, k, m Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (i), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (k), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (m). A total of n = 651 cells are quantified in this analysis. j, l, n Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (j), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (l), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (n). A total of n = 625 cells are quantified in this analysis.

    Techniques Used: Standard Deviation



    Similar Products

    brightline filters af488  (IDEX)
    90
    IDEX brightline filters af488
    a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing <t>DAPI</t> (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d-f Similar diagrams and images as (A-C), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g-h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Real data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and 2D KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’. i-n Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). Solid line is the mean and shaded area is the standard deviation. Dashed lines is the background fit. Dotted line is the colocalization by chance. i, k, m Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (i), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (k), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (m). A total of n = 651 cells are quantified in this analysis. j, l, n Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (j), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (l), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (n). A total of n = 625 cells are quantified in this analysis.
    Brightline Filters Af488, supplied by IDEX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/brightline+filters+af488/bio_rxiv__2025__01__05__631290-270-6-5?v=IDEX
    Average 90 stars, based on 1 article reviews
    brightline filters af488 - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing DAPI (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d-f Similar diagrams and images as (A-C), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g-h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Real data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and 2D KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’. i-n Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). Solid line is the mean and shaded area is the standard deviation. Dashed lines is the background fit. Dotted line is the colocalization by chance. i, k, m Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (i), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (k), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (m). A total of n = 651 cells are quantified in this analysis. j, l, n Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (j), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (l), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (n). A total of n = 625 cells are quantified in this analysis.

    Journal: bioRxiv

    Article Title: Transcriptional stochasticity reveals multiple mechanisms of long non-coding RNA regulation at the Xist – Tsix locus

    doi: 10.1101/2025.01.05.631290

    Figure Lengend Snippet: a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing DAPI (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d-f Similar diagrams and images as (A-C), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g-h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Real data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and 2D KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’. i-n Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). Solid line is the mean and shaded area is the standard deviation. Dashed lines is the background fit. Dotted line is the colocalization by chance. i, k, m Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (i), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (k), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (m). A total of n = 651 cells are quantified in this analysis. j, l, n Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (j), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (l), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (n). A total of n = 625 cells are quantified in this analysis.

    Article Snippet: Fluorescent signal was imaged with Semrock Brightline filters for DAPI (DAPI-5060C-NTE-ZERO), CY5 (NIK-0013 RevA-NTE-ZERO), TAMRA (SpGold-B-NTE-ZERO), and AF488 (FITC-2024B-NTE-ZERO).

    Techniques: Standard Deviation